RNAi for Plant Improvement and Protection

March 2021
More details
  • Publisher
  • Published
    15th March 2021
  • ISBN 9781789248890
  • Language English
  • Pages 216 pp.
  • Size 6" x 9"

RNA interference (RNAi) has the potential to make major contributions towards sustainable crop production and protection with minimal environmental impacts compared to other technologies. RNAi is being developed and exploited both within plants (i.e. host-induced gene silencing, HIGS) and/or as topical applications (e.g. spray-induced gene silencing, SIGS) for targeting pest and pathogen genes and for manipulating endogenous gene expression in plants. Chapters by international experts review current knowledge on RNAi, methods for developing RNAi systems in GM plants and applications for crop improvement, crop production and crop protection. Chapters examine both endogenous systems in GM plants and exogenous systems where interfering RNAs are applied to target plants, pests and pathogens. The biosafety of these different systems is examined and methods for risk assessment for food, feed and environmental safety are discussed. Finally aspects of the regulation of technologies exploiting RNAi and the socio-economic impacts of RNAi technologies are discussed.

1: Introduction to RNAi in Plant Production and Protection. Bruno Mezzetti, Jeremy Sweet and Lorenzo Burgos

2: Gene silencing to induce pathogen-derived resistance in plants. Zhen Liao, Elena Zuriaga, Ángela Polo and Maria L. Badenes

3: Exogenous application of small RNAs as a tool for gene function discovering. Barbara Molesini and Tiziana Pandolfini

4: The “Trojan Horse” approach for successful RNA interference in insects. Dimitrios Kontogiannatos, Anna Kolliopoulou and Luc Swevers

5: Biogenesis and functional RNAi in fruit-trees. Michel Ravelonandro and Pascal Briard

6: Gene silencing or gene editing: the pros and cons. Huw D Jones

7: Application of RNAi technology in Forest Trees. Matthias Fladung, Hely Haggman and S. Sutela

8: Host-induced gene silencing and spray-induced gene silencing for crop protection against viruses. Angela Ricci, Silvia Sabbadini, Laura Miozzi, Bruno Mezzetti and Emanuela Noris

9: Small talk and large impact: the importance of small RNA molecules in the fight of plant diseases. Kristian Persson Hodén and Christina Dixelius

10: dsRNA stability during external applications – an overview. Ivelin Pantchev, Goritsa Rakleova and Atanas Atanassov

11: Boosting dsRNA delivery in plant and insect cells with peptide- and polymer-based carriers: cases-based current status and future perspectives. Kristof de Schutter, Olivier Christiaens, Clauvis Nji Tizi Taning and Guy Smagghe

12: Environmental safety assessment of RNAi plants for pest control. Salvatore Arpaia, Olivier Christiaens, Paul Henning Krogh, Kimberly Parker and Jeremy Sweet

13: Food and feed safety assessment of RNAi plants and products. Hanspeter Naegeli, Gijs Kleter and Antje Dietz-Pfeilstetter

14: Regulatory aspects of RNAi in plant production. Wener Schenkel and Achim Gathmann

15: The Economics of RNAi technology in plant breeding: from the innovation landscape to consumer acceptance. Dario Frisio and Vera Ventura

16: Communication challenges of RNAi and selected communication messages from iPLANTA for dissemination. Hilde-Gunn Opsahl-Sorteberg

Bruno Mezzetti

Bruno Mezzetti has been a full professor since 2006 and director of the department 2009-2015, now Chair of the PhD school in Agriculture, Food and Environmental Sciences and of the UNIDO Second Level Master’s Degree on Biosafety of Plant Biotechnology, coordinator of 2 COST actions (COST836, COST863) and the FP7 project KBBE, EUBerry as well as having participated as a partner in 5 other EU projects. He is currently coordinator of a new COST action – iPLanta, and partner of a new Horizon 2020 Goodberry project. He has been coordinator and partner of several MIUR (PRIN) and Mipaf projects, as well as several projects with private companies operating in the nursery and fruit production sector. Prof. Mezzetti is author of a total of 156 publications in SCOPUS journals (IF:32) and several book chapters, topic review manuscripts and extension non-indexed publications. He has Chaired 4 ISHS international symposiums.

Jeremy Sweet

Jeremy Sweet is with the National Institute of Agricultural Botany (NIAB), Cambridge, UK.

Lorenzo Burgos

Lorenzo Burgos is with CEBAS-CSIC, Spain.

RNAi; SIGS; HIGS; dsRNA; miRNA; crop improvement; food quality; crop production; crop protection; biosafety; risk assessment; regulation