Testing Methods for Seed-Transmitted Viruses

Principles and Protocols

Spiral bound
January 2006
9780851990163
More details
  • Publisher
    CABI
  • Published
    5th January 2006
  • ISBN 9780851990163
  • Language English
  • Pages 268 pp.
  • Size 6.875" x 9.75"
$155.50

This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

PART I
Introduction
Seed transmission of viruses
Ecology, epidemiology and control
PART II
Biological assays
Serological testing methods
Nucleic acid-based testing methods
Nucleic acid hybridization
Epilogue
Appendix 1. List of seed-transmitted viruses and viroids
Appendix 2. Reagents, solutions and buffers
Appendix 3. Suppliers of laboratory equipment and materials

S E  Albrechtsen

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